Growth hormone insensitivity syndrome (GHIS) results from absent/attenuated growth hormone response on its receptor (GHR). It causes severe post-natal growth failure, hypoglycaemia, and obesity, high circulating growth hormone and low insulin-like growth factor 1 (IGF-1). We present two female siblings (S1 and S2) with GHIS due to the same novel mutation of the GHR and their response to IGF-1 over the first 3 years of treatment. S1 had normal size at birth (weight 3.46kg (77th centile), length 45cm (2nd centile)), but developed severe early growth failure, and was referred to endocrinology at 6 months of age. She had high circulating GH (67.5mIU/L, RI 6-30) and low IGF-1 (< 2.0 nmolL, RI 7.0-43.3). IGF-1 generation testing for suspected GHIS was consistent with the diagnosis (Table 1). Genomic sequencing detected a novel mutation of intron 4 of the GHR (GHR c.266+68T>G) with a predicted frameshift and early protein termination. S2 also developed post-natal growth failure due to the same GHR mutation. Both girls were commenced on IGF-1 treatment (Mecasermin 150mcg/kg/day) as a once daily s.c. injection (Laron, 2014). Blood and interstitial glucose monitoring were instituted. Growth velocities of S1 and S2 improved from 5.8cm/year (3rd centile) to 9.3cm/year (>97th centile) and 3cm/year (<3rd centile) to 7.7cm/year (>50th centile), respectively. The parents reported an improvement in energy, strength and appetite. Over the 3 years of treatment to date there have been ongoing improvements in height z scores from -6.6 to -4.0 for S1 and from -6.3 to -4.7 for S2. Despite dietary precautions, weight has increased disproportionately consistent with the GHIS phenotype, with current BMIs of 22.6kg/m2 (98th centile) for S1 and 16.5kg/m2 (76th centile) for S2. Hypoglycaemia has not increased on IGF-1 treatment. These cases highlight the important benefits of IGF-1 and difficulties encountered in the management of children with GHIS.