Invasive vertebrates pests continue to have devastating impacts on biodiversity and agricultural productivity in both Australia and New Zealand. Gene drive technology has emerged as a potentially powerful tool for suppressing invasive pest populations. By targeting genes for female fertility or development, suppression gene drives are more humane than conventional methods for pest control such as baiting, trapping and shooting. Our laboratory aims to demonstrate the feasibility of suppression gene drives through two parallel research endeavours. First, we are using the zebrafish as a vertebrate model to optimise the design of gene drives for their efficient propagation through a target population. Second, we are developing pipelines for producing animals of non-model vertebrate species (including fishes, amphibians and mammals) with targeted insertions of large DNA fragments using CRISPR.